Also, it may be a mistake to attribute all of your revenue from the email to email. By its nature, emails are going to the segment of your audience most interested and most likely to purchase. Only a portion of revenue from it is actually incremental; a large part probably would have ordered anyway, and the email gets credit only because they're also clicking through the email.
Of course, it depends on what offers are in the email, etc. If you want to test, the best bet is as was mentioned elsewhere in this thread, segment out a set of customers and change the frequency of emails over the long term, and track their total sales.
For example, our newsletters are always offering a time-limited discount for the purchase. They're also not segmented out to people "most likely to purchase", because we don't have that data. It goes out to 100% of our audience every time, unless they made a very recent purchase.
If we knew who the people are that are most likely to buy, it sure as hell would make the business easier to run.
(I’ve been blocking them for 25+ years; might be out of touch…)
In my experience most people do not block images in emails, and that's the easiest way to tell if someone opened your mail. Even most people who use an adblocker do not block email images.
click rates are probably a better proxy for this.
The unsubscribe rate doesn't really change, it's fairly constant at 0.3% of the audience every time.
There are obviously diminishing returns on the revenue with frequencies. Our business is moderately seasonal, so the numbers are not that accurate on the revenue side.