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Re:Whole Genome Sequencing by mbreese on Monday July 25, 2022 @06:33PM (#32231829)
Not to mention there is a good chunk of required "extra" equipment that is expected (centrifuges, etc) that bring the initial cost to well over $1000. You could buy most thing used, but some of the reagents could be difficult to acquire outside of a traditional lab.
Re:Whole Genome Sequencing by ejstronge on Monday July 25, 2022 @06:39PM (#32231899)
This is not strictly true - there is a transposase based prep that does not require special equipment.

Still, ONT users would need to understand how to convert raw reads into useable basecalls at loci of clinical interest

Re:Whole Genome Sequencing by mbreese on Monday July 25, 2022 @07:23PM (#32232281)
Does it still require a pipettor, or could it work with just a transfer pipet? Their field kit should probably be as minimal as it could be.

Last I looked into it, it was a minimal set of extra equipment -- votexer, centrifuge, etc... nothing unusual for a bio lab. And even most of that could be handled with a good snap of the wrist, if you weren't trying to be super precise.

Re:Whole Genome Sequencing by ejstronge on Monday July 25, 2022 @10:12PM (#32233459)
If one is patient, the only tool needed in the wet lab is a transfer pipettor and a scale - I think this could be done without a micropipettor