I guess yes, i.e. live performances where gameboy audio output was connected to a PA system[1] (likely together with other instruments like drum machines or samplers).

[1]: https://en.m.wikipedia.org/wiki/Public_address_system

"Live PA" is a term commonly used in the electronic music universe to describe a performance by an artist that typically involves all hardware (synthesizers, Gameboys in this instance, etc.) instead of all software or just someone DJ'ing music.

performing artist