nullbyte808

Did not even consider encrypted IRC as an alternative to Matrix or Signal. Or even running my own search engine. Good writeup! Very much for the 1%ers in tech skills.

I know, considering anyone can download music from youtube for free at any time. The same can not be said of books, before AA.

May as well just used pencil and paper. They would have to learn to improve their penmanship too.

"In the United States, if you’re lucky enough to have fiber, you might get 1 Gigabit. But often it’s shared with your neighbors."

Hey this can be good for privacy in a way at least. Your traffic can be from any of your neighbors. I wonder if you can sniff packets from other households?

Adorable! Maybe a personality that speaks in emojis?

I would bet in Avalonia UI. It's like WPF but cross-platform. https://avaloniaui.net/

Is it possible to get the reverse of this working? (Waydroid with play services on Linux phones, such as postmarketOS)

"A striking example of his forward-thinking occurred years ago on a beach in Ft. Lauderdale, Florida, where George-sitting on the sand with a laptop connected via his cell phone-became one of the first people to read email over a mobile connection to a computer at Purdue. As a friend noted, "This was a real bit of history… At the time Apple had a whole engineering team trying to do this and here's George on the beach making it happen.""

Amazing. RIP.

Dear god how do I get these jobs? I'm 35 yo and would work with you and accept your work, not jam crap code into things. I'm open minded and realize when someone's idea or code is better than mine.

Hypothetically what would happen if China took over Tawain and TSMC?

I wonder if the vale of Pixel phones that run GrapheneOS will rise dramatically?

Malware could still use DNS records for storage and access to bootstrapped payloads correct?

Does this work?

I almost switched back to Fedora Bazzite to get a working gamescope, but realized I can get HDR in sway and its actually more stable than Valve's mess of gamescope. Even though I have to use "--unsupported-gpu" flag, my Nvidia card works wonders in Sway, where as gamescope gives me a blinking cursor and segfaults.

Some things are not that simple and nix options come in handy automating other packages and services needed.

also the AI hallucinating nix options. I have to constantly check https://search.nixos.org/packages?channel=unstable

I recently fixed the pipewire audio stutters by just giving gemini my flake and asking how to fix it. It suggested a few fixes and low and behold they were gone! Here's my flake with impermanence + yubikey login: https://github.com/leonewt0n/nixos

How do you know its AI? Who cares, sounds fine too me.

1. Makes no sense. 2. not true. 3. You can turn features you don't like off, like the AI 4. False. The bootloader is not locked. Linux does work, but it would be nice if they activly worked on kernel modules for their hardware. 5. Macbooks have the longest shelf life of any consumer PC. Period.

Like saying my PC boots up 2x faster so it must be 2x more powerful. lol

Commenting from the app. Good job!

The text details the methodology for "Sidewinder," a DNA assembly approach that utilizes oligo-derived heteroduplex fragments, barcode sequences, and toehold-mediated ligation. The process relies on computational design (NUPACK), specific thermal cycling protocols, and rigorous sequencing validation to assemble and screen complex DNA constructs.

Fragment and Barcode Design

Sidewinder fragments are composed of DNA oligos. The design process relies on calculating the length of a construct and a barcode to determine the maximum bases of coding information.

Toeholds: Standardized to 10 bases. Ligation is effective at ≥±6 bases from the Sidewinder helix; 10 bases allow sufficient distance for ligase docking.

Barcodes: Designed to be compatible with toeholds using a "guess-check" method or NUPACK Python scripts. Barcodes are screened for secondary structure and crosstalk at 50°C.

Ligase Selection: Taq ligase and HiFi Taq ligase were selected for their stability at high temperatures and efficiency at the 3-way junction (3WJ).

Oligo Preparation and Annealing

Sourcing: Oligos were synthesized by Millipore-Sigma. Standard desalt purification was generally sufficient, though PAGE purification was used for specific characterization steps.

Heteroduplex Formation: Coding oligos (phosphorylated via T4PNK) and barcode oligos are annealed in a PCR tube. The mixture is heated to 98°C and ramped down to 25°C to form stable heteroduplexes.

Purification: Annealed heteroduplexes are purified via PAGE gel extraction.

Assembly Protocols

The authors describe two primary assembly conditions using HiFi Taq ligase with fragments at equimolar concentrations (~1 nM):

Cycling Protocol: Cycles between 85°C (1 min) and 50°C (2 min) for 100 cycles, followed by a 1-hour hold at 50°C.

Ramp Protocol (e.g., h-fibroin): Heats to 85°C, slowly cools to 50°C, and incubates overnight at 50°C.

These methods were benchmarked against conventional 2-way junction (2WJ) and Gibson assembly methods.

Such a simple concept took this long to discover? Now we just need a way of packing the DNA strings into blank cells reliably.

DOJ are technically breakng the law by releasing a heavily moddified "reproduction" of the original files, not the "actual" files. The software they used "OmniPage CSDK 21.1" removes all usefull metadata and any encrypted files if any where stored.

I have a hybrid approach with GrapheneOS. 99% of the time I only use WiFi on my phone via a Tor router. I have an anonymous KeepGo ESIM with global data that does not expire and use it when I have to when Im away from home.

Hippies...