No. Checks clear in 24 business hours, and fedwire wire transfers work same day if they are submitted early enough, and in no case more than 24 business hours (for domestic wires). Oftentimes domestic fedwire transfers are nearly real-time, and there is a plan for further modernization to speed things up in more cases.
ACH transfers are also 24 business hours.
The check clearing thing is called Check 21 and it was a big thing back in 2004 when it went into effect.
https://www.federalreserve.gov/paymentsystems/regcc-faq-chec...
What? The third-world banking system of the US often takes MUCH longer than that.