Re:Scientists found genetic mutations in every astronaut blo...
by jostmey on Friday September 09, 2022 @12:21PM
(#32781177)
DNA sequencing wasn’t an economically feasible option 20 years ago
Re:Scientists found genetic mutations in every astronaut blo...
by 1970-01-01 on Friday September 09, 2022 @12:26PM
(#32781278)
OK 20 years maybe it wasn't worth the cost, but by 2011 it was only thousands of dollars.
https://www.genome.gov/about-genomics/fact-sheets/DNA-Sequen...
Re:Scientists found genetic mutations in every astronaut blo...
by bergenty on Friday September 09, 2022 @04:20PM
(#32784392)
Well you’re not addressing the GPs concerns. The DNA could be severely damaged.
Re:Scientists found genetic mutations in every astronaut blo...
by anarticle on Friday September 09, 2022 @01:29PM
(#32782307)
Ouch, it is not uncommon to freeze samples for a long time, especially rare ones like this. You're free to peruse the literature on how this is an accepted practice.
Re:Scientists found genetic mutations in every astronaut blo...
by 1970-01-01 on Friday September 09, 2022 @03:00PM
(#32783463)
So I did. Searched and read about it, found out the viability and recovery of cryopreserved PBMC has a clear downward slope. Samples are good for years, not decades.
Re:Scientists found genetic mutations in every astronaut blo...
by anarticle on Saturday September 17, 2022 @02:29AM
(#32875249)
This depends on what you want to do. The DNA can certainly be sequenced. They might even be viable if you’re nice to them.
What I mean to say is this is an accepted practice in many labs, including ones I have worked in. In my experience, these were old tumor cells stored in LN2. Sources include: https://link.springer.com/article/10.1007/BF01131376
So "scientists" stored DNA for 20 years and then decided to take a closer look? Better than nothing, but not by much.