Absolutely. I've done a lot of interviews for different companies, and it is clear when someone is asking a pre-canned list of questions vs. someone who has real questions and genuine interest. I wouldn't give a no-hire recommendation just because someone did that, but it is definitely off-putting. Genuine questions asked out of interest can lead to an interesting discussion which could give a boost to a candidate. (Obviously, actual skills are the most important thing, but when choosing between a couple of candidates, both seeming fits for a role, it could tip the scales.)