Sure, but by the time I would call someone my friend or acquaintance I would assume that they would feel safe enough saying anything that might reveal that fact in an indirect way. Then again, being gay/lesbian is very much an accepted thing here. Being trans still not so much, though that is changing.

If I would meet some stranger or some person in a work setting their sexuality would not be on my list of things I would be interested in, either we don't know each other that well or we have at best a working relationship, a job to get done, and to move the subject to sexuality would be - according to me - inappropriate.