Re:CRISPR Editing in Primates
by ChaitanyaSai on Thursday May 20, 2021 @02:32PM
(#27225195)
Thanks for the pointers! A big fan of birds-eye-view books that let readers then follow individual trees. I guess these take time to put together and some fields (like genomics / genetic engineering, are too fast moving now?)
> A big fan of birds-eye-view books
Not a book, but the News Features in Nature have a similar flavor, eg [1]. And for following up with the motivating article... well, there's sci-hub, for now.
[1] "The mysterious microbes that gave rise to complex life" https://www.nature.com/articles/d41586-021-01316-0 in https://www.nature.com/nature/volumes/593/issues/7859 .
Re:CRISPR Editing in Primates
by Duller-Finite on Thursday May 20, 2021 @10:32PM
(#27229894)
The Code Breaker by Walter Isaacson is a recent biography of Jennifer Doudna, who won the Nobel Prize for her pioneering work on CRISPR, that you might enjoy
Re:CRISPR Editing in Primates
by alextheparrot on Saturday May 22, 2021 @03:06PM
(#27249381)
I’ve been recommending The Gene to people who are just generally interested in this sorta thing
Most of the targeting today is happening through antibodies, but the majority of LNP delivery at this point is done passively without targeting mechanisms on the LNP (Needle injection point aside).
That's actually one advantage of CRISPR over just injecting mRNA - you can target specific cell-lines (Even if you change the genome of many cell types) by using cell-specific promoters for the edits you make instead of relying on surface affinity based targeting of the LNP.
Interesting resources:
[0]: https://www.nature.com/articles/nrd.2017.243 (General review 1)
[1]: https://www.frontiersin.org/articles/10.3389/fmolb.2020.5879... (General review 2)
[2]: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5498813/ (PCSK9 target again, but via siRNA actuator)
[3]: https://genomebiology.biomedcentral.com/articles/10.1186/s13... (Example of cell-specific CRISPR integration)