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Re:OpenFlexure Microscope, an open-source optical microscope by JackMcMack on Thursday May 20, 2021 @05:47AM (#27219234)
I'm certainly no expert, my knowledge is limited to what I picked up after a few nights of reading about the microscope.

Does the beamsplitter have to be non-polarizing? My understanding is that standard beamsplitters do the splitting by polarizing the incoming light.

Now that I think of it, you're probably using a standard display as your image source? That does indeed produce polarized light. In that case I would try a (common in photography) circular polarizing filter. Rotate the filter to match the display polarization, after that the light will be circularly polarized. I think you could use that in combination with a "standard" polarizing beamsplitter. Depending on the desired dimensions, there are a few available on aliexpress.

Re:OpenFlexure Microscope, an open-source optical microscope by DoingIsLearning on Thursday May 20, 2021 @07:01AM (#27219622)
Thanks for the pointers, I had assumed non-polarized was a requirement but indeed didn't consider that display sources are themselves polarized.