Switch it to a fireworks show and watch the quality suffer.
320kbps MP3 just means the codec allocates 320kb to store each second of compressed signal. Obviously some signals compress better than others and need much lower than that for an acceptable quality (audiobooks are often distributed at 64kbps).
And as I said, the airpods are medium quality earbuds. Not bad, not great. You will NOT hear any loss of audio quality if you play 320 kbps mp3 through them.
I'm sure most people could not hear any quality loss on 128 kbps mp3 (I don't these days), or even 64. But above 320 there is just no way.
The practical difference in the listening experience is, as you infer, negligible, to almost everyone. Classical music, or other such audio with large dynamic ranges, suffer the most. And while those frequencies above 12-16kHz are often the most obviously hit, the entire range suffers in other ways, phase, low end smearing, transient loss. And while first generation lossless to lossy conversion really is an excellent trade off, lossy to lossy very quickly produces audibly worse sound, even 320 to 320 conversion. But you knew that.
It’s not like anything above 320 kbps mp3 will be detectable.