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A comment on I should have loved biology. Parent comment

Re:I should have loved biology by dekhn on Thursday November 19, 2020 @10:06AM (#25150234)
AFM isn't really used in biology.
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Re:I should have loved biology by infinite8s on Thursday November 19, 2020 @10:32AM (#25150505)
But it is used a lot in biophysics!
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Re:I should have loved biology by dekhn on Thursday November 19, 2020 @10:58AM (#25150760)
It is? I mean, that was my PhD, but I don't recall anybody ever using AFM for biology. Huh, apparently now they do.
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Re:I should have loved biology by infinite8s on Thursday November 19, 2020 @11:33AM (#25151153)
Yeah lots of single molecule studies (strength of an actin filament, how much force needed to 'unfold' various proteins, etc). And this was 20 years ago when I was in grad school. Haven't kept up with the state of the art today.
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Re:I should have loved biology by dekhn on Thursday November 19, 2020 @01:13PM (#25152428)
interesting. all the people doing that in my day (also 20 years ago) used fluorescent microscopes (Ron Vale) with labelled protein. I can't see how you could unfold a protein using AFM, since it's in vacuum state, although I totally understand how optical tweezers would be fine to do this in solution.
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Re:I should have loved biology by nynx on Thursday November 19, 2020 @01:15PM (#25152439)
AFMs work in both air and fluid environments.
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