This assumes, of course, that the PCIe lanes in question actually exist† and are dedicated to the card, and are not shared with another in-use device via a switch or similar chipset shenanigans.
Case in point: the only time I ever had performance problems with my eGPU setup was when attempting to do 4K HDMI output through a Blackmagic Decklink card while simultaneously using the 1070Ti for compute over the same (shared) Thunderbolt bus in DaVinci Resolve.
As far as I know, this would not typically be a problem with a single-GPU, non-Thunderbolt-connected desktop system, as desktop motherboards typically have at least one full-bandwidth x16 slot, which you'd almost always use for the GPU.
† Physical x16 slots can have fewer than sixteen connected PCIe lanes. For example, while my HP Z820 has four "x16" slots, only three have full bandwidth; the fourth slot supports x16 cards, but only at the speed of an x8 slot, because only eight of the slot's sixteen PCIe lanes are actually connected.
There are also some sites that explains why large capacity power suppliers are a waste of money. Ex: https://www.youtube.com/watch?v=X_wtoCBahhM
About the PSU, for me the only reason to go with a 1000W PSU is that the fan will remain quiet with a GTX 1080 when idle and gaming as well. At least thats what I hope.
If the same is possible with the 3080 is more questionable, especially if the card draws 320W at all times.
PSU efficiency is low at the ends of the curve, underutilized (<20%) the efficiency can drop as low as 70%. The top (not peak) efficiency is somewhere in the 30-80% load zone, that is where you want to be.
Based on that info and that the system will draw about 430W for CPU+GPU, it seems that the fanless Seasonic TX-700 is better suited.
It is quite expensive, but as someone who really hate unnecessary noise, it looks like a good choice.
Changing all the existing cables to a new PSU sucks though :p
If you have a spare core not being used when playing a video game, it can be used which should make the difference between 3.0 and 4.0 somewhat indistinguishable.
Looking forward to some benchmarks that evaluate the difference between PCIe 3.0 and 4.0. Getting a new motherboard with PCIe 4.0 along with a CPU will up the cost considerably, so hopefully that can be avoided.
Also considering an upgrade to the Corsair RM1000x PSU which should be able to be totally silent even with the RTX 3080 according to https://www.tweaktown.com/reviews/7376/corsair-rm1000x-1000w....
UPDATE:
According to this answer, using PCIe 3.0 should not make a huge difference: https://www.reddit.com/r/nvidia/comments/iko4u7/geforce_rtx_...