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Re:Nanopore sequencing, Shasta toolkit enable de novo assemb... by z991 on Tuesday May 05, 2020 @07:36PM (#23086339)
We used the rapid kit and also a gelbox / agarose.
Re:Nanopore sequencing, Shasta toolkit enable de novo assemb... by vikramkr on Tuesday May 05, 2020 @08:59PM (#23086883)
Did you end up buying everything? I'd be curious to know how much it cost overall if you'd be willing to share. While most of my time has been in well stocked academic labs or shared startup lab space, I did spend some time working out of a bio hacker space and I'm always curious how cost efficiently people can wrangle together enough otherwise expensive kit to run an experiment in an odd setup. Did you end up with a lot of extra agarose etc or were you able to resell the leftovers and the used kit? Super cool stuff.
Re:Nanopore sequencing, Shasta toolkit enable de novo assemb... by z991 on Wednesday May 06, 2020 @12:46AM (#23087985)
In total it was about $3k. So not cheap, but also not a real lab budget by any means.