No human genome has ever been completely sequenced

Re:No human genome has ever been completely sequenced by iskander on Tuesday October 24, 2017 @08:29AM (#15541048)

Are those experiences on recent chemistries/flow cells? My first library prep gave me ~4 gigabases on a R7.4 flow cell and that was pretty low compared with everyone else in the workshop.

Also, which library prep are you using? I think the ligation kit has historically gotten better yield (not sure if that's true any more).

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Re:No human genome has ever been completely sequenced by daemonk on Thursday October 26, 2017 @02:40PM (#15561735)

Are you working on cell lines or bacteria? Genomic dna? I find most people that tell me they get good data yield are working on samples where dna extraction is relatively straightforward and dna yield is abundant.

I used the flowcell version previous to the current one and got ~2gb of data. I used the ligation kit on genomic extraction of ~10 individuals (crustacean).

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Re:No human genome has ever been completely sequenced by aroch on Tuesday October 24, 2017 @10:10AM (#15541627)

The new rapid kits (R3) have significantly worse performance than the R2 kits it seems.

I'm also extremely put off by how they handle support -- that is via a forum where they do their very best to barely answer anything

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Re:No human genome has ever been completely sequenced by daemonk on Thursday October 26, 2017 @02:42PM (#15561755)

I agree about their communication and support. It does seem like they put all their support behind the few big labs in the UK that are actively using nanopore (free R&D for them I guess). I get a sense of "if you can't get this to work, then you are just a bad scientist" vibe from them.

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