I think recently, another interesting proof for this is the Joker's note. Watching the Dark Knight behind the scenes commentary, Hans Zimmer talks about how he thought the most chill-inspiring theme for Joker wouldn't be an intricate composition, but one slightly sour note played at length. Other sounds and effects were layered upon it to fit with the soundtrack, but the effect is amazing, simultaneously warning the audience of the joker's presence and inspiring suspense at the same time.