Re:Researchers Show How Lost Sleep Leads to Lost Neurons in ...
by sillysaurus3 on Wednesday March 19, 2014 @05:12PM
(#7431898)
Yes, the PDF is paywalled. Would you mind posting a Dropbox link to it or emailing it to me? Thanks!
This is a very interesting paper, but I need to analyze the specific method they used to keep mice awake. All that paragraph says is "we used a previously validated technique," with no details as to how specifically they woke the mice up.
Re:Researchers Show How Lost Sleep Leads to Lost Neurons in ...
by treePhase on Wednesday March 19, 2014 @06:19PM
(#7432270)
I believe this is the cited paper: http://www.jneurosci.org/content/30/43/14543.full.pdf
They describe the "novel environment" starting on page 3.
I'm on an academic network, so I'm not sure if the PDF's paywalled...
From a cursory glance at the paper, this is what they had to say about how they kept the mice awake:
Mice were housed in a light/dark environment with lights on from 6:00 A.M. to 6:00 P.M. ... A previously validated, enriched, novel environment (Gompf et al., 2010) was used to promote spontaneous exploratory wakefulness. The short-term wakefulness (Sh Wake) period selected was 3 h during the lights-on period. With zeitgeber time 0 h (ZT0) referenced to the onset of the lights-on period, Sh Wake occurred at ZT8–ZT11, while extended sleep loss (Ext Wake) consisted of 8 h of continuous wake time at ZT3–ZT11 with 16 h intervals in the home cages after the first and second days of 8 h wake time.