Re:Invention of DNA "Page Numbers" Opens Up Possibilities fo...
by zulko on Friday February 06, 2026 @11:55AM
(#46915212)
Possibly not what you're asking for, but I wrote a generally-accessible intro to why it can be tricky to assemble many DNA fragments with "Golden Gate Assembly", a mainstream method which relies on short sequence overhangs. The Sidewinder method discussed in this thread aims to solve that "short overhang" problem.
https://zulko.github.io/bricks_and_scissors/posts/overhangs/