Can you tell more about recreational nitrous oxide and when does the "damage occur"? Is there the same thing with wim hof? (like for example with oximeter 80 Sp02 or below?) I got in wimhof/oxide around 80 Sp02 the interesting thing is I got this feeling with fighting to hold my breath but below 90 I kinda needed to convince myself that I should breath in both cases,
So while interesting as a study, I don't think it offers any insight into the kind of breathwork described in the nature study.
I did a longer write-up on the physiological effects which you might find interesting: https://docs.google.com/document/d/1RuDv_E9osM1CCFWZMywMru9J...
Long story short, your neurons get just a tad bit more excitable because calcium that usually acts like the bouncer to the hot club is busy snogging albumin. That has very little effect in places in the body, but in motor neurons that control your smallest muscles (face and hand), and in sensory neurons under your skin it does move the needle — that causes the muscles to contract and your skin to feel tingly, both exactly the same cause.
This is the reason people with epilepsy should _NOT_ do breathwork, but for otherwise healthy adults there are no negative long term effects of respiratory alkalosis — a few normal breaths to balance out your co2 and the symptoms will go away.
[1] "Brain Damage in Commercial Breath-Hold Divers" https://journals.plos.org/plosone/article?id=10.1371/journal...
[2] "Do elite breath-hold divers suffer from mild short-term memory impairments?" https://cdnsciencepub.com/doi/10.1139/apnm-2017-0245
Ref. [2] is especially concerning to me in pushing in any sort of static apnea training or breathwork: "The time to complete the interference card test was positively correlated with maximal static apnea duration (r = 0.73, p < 0.05) and the number of years of breath-hold diving training (r = 0.79, p < 0.001)."