Re:Want to build a sequencer? 454.bio opens up their plans
by aabaker99 on Tuesday February 06, 2024 @07:12AM
(#39273403)
If you’re up for reading a dissertation I have one in mind I can dig up. I will check back here in 12 hours or so or you can contact me from my profile.
Re:Want to build a sequencer? 454.bio opens up their plans
by bettercaust on Tuesday February 06, 2024 @09:29AM
(#39274545)
Would also be interested in this if you'd care to post publicly.
Re:Want to build a sequencer? 454.bio opens up their plans
by anonymouskimmer on Thursday February 08, 2024 @12:54PM
(#39305130)
In case you missed it: https://news.ycombinator.com/item?id=39283253
Re:Want to build a sequencer? 454.bio opens up their plans
by anonymouskimmer on Tuesday February 06, 2024 @12:10PM
(#39277067)
That sounds great.
Re:Want to build a sequencer? 454.bio opens up their plans
by aabaker99 on Tuesday February 06, 2024 @08:15PM
(#39283253)
It turns out the dissertation is not quite published yet, but this paper [1] will be a part of it. Sam and I were in the same lab and his work has evolved to focus even more on AI-guided protein design. Perhaps the quote from the abstract that made the connection for me to this post:
> We applied the protein G B1 domain (GB1) models to design a sequence that binds to immunoglobulin G with substantially higher affinity than wild-type GB1.
[1] https://www.pnas.org/doi/pdf/10.1073/pnas.2104878118?downloa...
Re:Want to build a sequencer? 454.bio opens up their plans
by anonymouskimmer on Thursday February 08, 2024 @12:53PM
(#39305126)
Thank you very much!
Any recommendations on learning how to do this? As well as the limitations to your approach? I've been interested in engineering site-specific DNA binding proteins for over a decade now, but the tools I've looked at are specific for protein to small-molecule or protein to protein interfaces.