A GTK 2 application compiled 25 years ago was before the Big Glibc Switch that caused us so many headaches in the early 00's, so no, it won't.
My xv copy has a mtime of Aug 4 1998 (pre-compiled binary from the RPM on the website). It runs on my current Void Linux system. Not GTK, but that's a detail: point is it runs, and is 25 years old. I've also run old Opera copies from the 90s, although that's longer ago and I'm not sure if that still works.
*Usually, not always.
A GTK 2 application compiled 25 years ago usually still runs today, too. Just because GTK 3 is available doesn't mean GTK 2 stopped existing.