You can have two tracks of 1-1s. You do the layoffs and pick a lieutenant to do 1-1s with the stayers. They should be able to get through those much quicker so you can leave your go forward team with as little purgatory time as possible. I would suggest prioritizing that list by how worried you think they’ll be as an additional attempt at humanity.

One other note: you are transitioning company size. You were on the cusp of midsize and you are right back to startup. Make your choices accordingly. Even if someone was going to be great at 50->100 they may be the wrong person for 25. Keep the scrappy, true-believer do-ers. You’ll need them.