Re:Gene Therapy Reverses Biological Aging by Six Years
by Pigalowda on Wednesday June 01, 2022 @08:11AM
(#31580679)
The DNA injected is a plasmid with a myosin promoter supposedly introduced via electroporation through the call membrane and then presumably into the nucleus.
Successful electroporation of large DNA fragments is difficult (31aa here = 93bp). Widespread DNA uptake, nuclear localization, and subsequent integration is a low probability event.
Another barrier from what I understood working with transgenics is that Plasmid DNA is a foreign DNA morphology outside of our mitochondria. It is the shape of bacterial DNA and initiates a host response to destroy it. That could be old/incorrect understanding from myself though.
Another thing which bothered me was there was no discernible increase in growth hormone. Activation of GHRH should lead to increase in growth hormone? Perhaps Somatostatin activity is regulating away the increase.
Anyways, I’m skeptical of successful DNA integration and therefore the beneficial claims. The subject isn’t living in a bubble, there will be cofounders and lifestyle changes over 5+ years.
Successful electroporation of large DNA fragments is difficult (31aa here = 93bp). Widespread DNA uptake, nuclear localization, and subsequent integration is a low probability event.
Another barrier from what I understood working with transgenics is that Plasmid DNA is a foreign DNA morphology outside of our mitochondria. It is the shape of bacterial DNA and initiates a host response to destroy it. That could be old/incorrect understanding from myself though.
Another thing which bothered me was there was no discernible increase in growth hormone. Activation of GHRH should lead to increase in growth hormone? Perhaps Somatostatin activity is regulating away the increase.
Anyways, I’m skeptical of successful DNA integration and therefore the beneficial claims. The subject isn’t living in a bubble, there will be cofounders and lifestyle changes over 5+ years.