CRISPR Editing in Primates

Re:CRISPR Editing in Primates by elihu on Thursday May 20, 2021 @06:59PM (#27228507)

I'm not an expert so maybe someone can give a better response, but I've understood CRISPR as something that doesn't necessarily edit all the targeted cells, just some proportion of them. So, maybe if one doesn't want to eliminate the production of a compound entirely, one sets the dose low enough to to only edit the genes of, say, half the targeted cells.

If I'm interpreting the article correctly, though, it sounds like in their trials they think they may have edited nearly all the targeted cells.

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Re:CRISPR Editing in Primates by f6v on Friday May 21, 2021 @03:15AM (#27231336)

> one sets the dose low enough to to only edit the genes of, say, half the targeted cells

Using the single-cell sequencing we’ve learned that disease is sometimes driven by a very small sub-population of cells(e.g. cancer treatment resistance). There’s no way of knowing how the organism will respond to a perturbation even in 1% of the cells.

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