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A comment on Moderna mRNA sequence released to GitHub [pdf]. Parent comment

Re:Moderna mRNA sequence released to GitHub [pdf] by subroutine on Monday March 29, 2021 @08:17PM (#26629948)
Meh, they probably just used lipofectamine (which has been around since the 90s) or something very similar.

https://en.wikipedia.org/wiki/Lipofectamine

https://www.thermofisher.com/us/en/home/brands/product-brand...

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Re:Moderna mRNA sequence released to GitHub [pdf] by Duller-Finite on Monday March 29, 2021 @10:57PM (#26630858)
lipofectamine is used for in vitro transfection, not in vivo gene delivery. The vaccines use lipid nanoparticles rather than liposomes
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Re:Moderna mRNA sequence released to GitHub [pdf] by neuronic on Tuesday March 30, 2021 @04:36AM (#26632333)
I absolutely do not have a link, but I remember reading that the lipid nanoparticles are actually created by mechanical action (possibly fluid dynamics/turbulence). I thought that was pretty neat.
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Re:Moderna mRNA sequence released to GitHub [pdf] by MuffinFlavored on Monday March 29, 2021 @11:11PM (#26630933)
If they didn't use lipofectamine, what did they most likely use?
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Re:Moderna mRNA sequence released to GitHub [pdf] by flobosg on Tuesday March 30, 2021 @12:21AM (#26631239)
Derek Lowe discussed it a few months ago:

https://blogs.sciencemag.org/pipeline/archives/2021/01/11/rn...

https://blogs.sciencemag.org/pipeline/archives/2021/02/22/mo...

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