Re:Super Resolution
by nnmg on Thursday March 18, 2021 @01:59PM
(#26505529)
I am least familiar with SIM, but you can do live-cell SIM imaging for sure. The processing is a bit computationally intensive but not so bad (and can always be done post-hoc).
The big thing you have to look out for is the light intensity killing the cells or bleaching your signals. One of our collaborators is actively working on on-the-fly SIM processing for live cell imaging.
The big thing you have to look out for is the light intensity killing the cells or bleaching your signals. One of our collaborators is actively working on on-the-fly SIM processing for live cell imaging.
A quick glance at pubmed it looks like 11Hz was do-able several years ago https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2895555/ and this (sorry paywalled, I've heard sci-hub has the paper...) https://pubmed.ncbi.nlm.nih.gov/30478322/
STED is promising for live imaging too. Lots of beautiful pictures out there!