I don’t expect, yet also wouldn’t be surprised if, an Islamic fundamentalist group genetically modifies a pig-specific retrovirus so that any pig which catches it will start internally producing enough capsaicin that nobody will even want to eat pork.

I do expect software based attacks to achieve government goals.

I would be only a little surprised by private nukes or lunar mass drivers.

If mind-uploading becomes a thing, I expect totally novel forms of interrogation in addition to virtual hells (a la Surface Detail by Iain M Banks), and several other legal and political messes outside the scope of this thread.

3D printing, protein engineering, AI controlled mining robots, etc. will lead to synthetic von Neumann machines that radically transform industry, giving small states or mid-sized criminal gangs significant war-fighting capacity.

AI can out-strategise humans. Who trusts it, wins — unless the AI was made wrong, in which case who trusts the AI, loses. Nobody will know if the AI is right or not until too late.

A/B testing propaganda, so perhaps you can fool all of the people all of the time.

Most of these are better as Hollywood plots than as real concerns. Whatever does eventually happen will seem obvious after the event, but will seem like science fiction right up until that point.