Re:Sequencing your DNA with a USB dongle and open source code
by searine on Wednesday February 03, 2021 @04:19PM
(#26019245)
They mean at genome-wide scales. If you are just doing a 410bp the sequence is short enough that the signal of is going crush and noise you get from strands slipping in the pores.
The errors nanopores get are gaps, not base pair substitutions. So with things like viral or bacterial sequencing you don't really have huge issues.
When you are doing large eukaryotic sequences with lower coverage on average, you start picking up a lot of deletion artifacts. Which isn't a huge deal if you have a very well annotated genome like human, but if you are doing pioneer genomics it can create some difficulties. Often if the genome isn't well annotated, its best to pair nanopore with short reads.
The errors nanopores get are gaps, not base pair substitutions. So with things like viral or bacterial sequencing you don't really have huge issues.
When you are doing large eukaryotic sequences with lower coverage on average, you start picking up a lot of deletion artifacts. Which isn't a huge deal if you have a very well annotated genome like human, but if you are doing pioneer genomics it can create some difficulties. Often if the genome isn't well annotated, its best to pair nanopore with short reads.