Sequencing your DNA with a USB dongle and open source code

Re:Sequencing your DNA with a USB dongle and open source code by staplung on Wednesday February 03, 2021 @03:02PM (#26018423)

Unfortunately, with nanopore the errors are biased so you tend to get errors in the same places. All sequencing techniques also have error rates but some are unbiased so running a single sample through (which will usually have many, many copies of any sequence) will average out to a good read of the sequence.

Some good info on next-gen sequencing techniques: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3841808/

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Re:Sequencing your DNA with a USB dongle and open source code by tdido on Wednesday February 03, 2021 @03:26PM (#26018658)

Still, some of the errors can be compensated for with more coverage. So if you can manage 20-30X you're left with the homopolymer problem (nanopores can't tell how long a stretch of the same repeated nucleotide is, because you can't control how long the sensed kmer stays in the pore), but lots of other types can be improved quite a lot.

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