Positions like 172 have errors more often than not because the basecaller is wrong sometimes (note: this is from a sequence verified sample).
The errors come up more often in some sequences than they do in others. I’m not really sure about symbol processing, but if you have any beginner resources for that I’d appreciate them!
There is a real example I ran a few months ago. How to read it is here https://en.m.wikipedia.org/wiki/Pileup_format
Positions like 172 have errors more often than not because the basecaller is wrong sometimes (note: this is from a sequence verified sample).
The errors come up more often in some sequences than they do in others. I’m not really sure about symbol processing, but if you have any beginner resources for that I’d appreciate them!