Because they didnt want air going in or out of the biosphere they had to figure out a way to account for the massive change in volume as the air temperature inside changes between super hot days and cold nights, otherwise you'd blow the windows out of the thing during the day.
Shocking ...
Including humans seems to have caused most of the problems there. If you actually wanted to experiment with closed biological systems in a useful way then I'd recommend doing a bunch of trials with a less cantankerous species of large omnivorous mammal, like the goat.
>including humans seem to have caused most of the problems
I chuckled a bit at your reply hvd.
The hardest, seemingly intractable problems in the world aren't engineering problems, they are human ones. If we want to level up as a species those are the ones we will need to figure out how to solve.
https://en.wikipedia.org/wiki/Biosphere_2