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Re:A new and improved Twitter API by jdminhbg on Friday July 17, 2020 @12:07PM (#23872017)
I'm not sure which hack Tweetbot is using to do this (I think search as noted below), but you can tell it's a hack because it only works for recent-ish tweets. If you go to a tweet a couple weeks old and view details, you can see that it won't fill in the replies.
Re:A new and improved Twitter API by jeromegv on Friday July 17, 2020 @01:40PM (#23873229)
Can confirm, Tweetbot never has "all" the replies.
Re:A new and improved Twitter API by roberto on Friday July 17, 2020 @04:13PM (#23874965)
The way clients usually do this is by fetching all mentions to the user after a given timestamp (of the original tweet), and collecting only those that are replies to the tweet. Since there's a limit on how many mentions you can get, you might not get all the replies.

It's been like that at least since 2013.