I'm not so much referring the the political problem of inhabiting the area, as to the technical and logistical problem of having a near closed loop environmental system. Sure people can and do live in northern Mali or the southern Libyan desert but even those small towns are connected to somewhat established networks of cargo truck delivery.

Set up a colony of 50 people in bir tawil and measure how much outside water, food and other supplies they need (similar problem to biosphere 2) in kg per person per year... And that's in a place where you can extract some water from air moisture, with difficulty , and you can breathe the air outside.