Re:US admits 'failing' on testing, says Fauci
by chvid on Thursday March 12, 2020 @01:27PM
(#22559309)
I thought this polymerase chain reaction test was something you could do at any university hospital?
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Re:US admits 'failing' on testing, says Fauci
by jfarlow on Thursday March 12, 2020 @01:32PM
(#22559375)
From a technical perspective, it is a simple test. Hard part is chain of custody and sample integrity - controls, etc.
Re:US admits 'failing' on testing, says Fauci
by heyitsguay on Thursday March 12, 2020 @01:33PM
(#22559388)
Are there any differences for Covid-19 samples versus any of the other samples such labs would be routinely testing though?
Re:US admits 'failing' on testing, says Fauci
by chromatin on Thursday March 12, 2020 @01:37PM
(#22559441)
Yes, it is RNA virus so it is an extra step that is not necessary in many other PCR based clinical tests.
Re:US admits 'failing' on testing, says Fauci
by heyitsguay on Thursday March 12, 2020 @01:49PM
(#22559599)
Sure, I meant for the non-technical aspects that the previous poster mentioned - chain of custody, sample integrity.
Re:US admits 'failing' on testing, says Fauci
by jfarlow on Thursday March 12, 2020 @03:16PM
(#22560641)
So the process used in this testing is so common that research labs and any biochemistry lab in any college or high-school can run the test. But they probably shouldn't because they have no practice nor experience providing a diagnosis to a human - much less the strict statistical controls to interpret their results reliably.
A clinical lab that is used to running these kinds of (very common) protocols will see no difference in this protocol from others they commonly run (from a chain of custody, sample integrity, etc. perspective), just with a slight change to their reagents to specifically test for COVID. Their handling should be no different.
Re:US admits 'failing' on testing, says Fauci
by chromatin on Thursday March 12, 2020 @01:37PM
(#22559434)
It is, but there have been major bureaucratic delays, and no one was willing to step up and say "forget it, we are doing it". In addition, there have been shortages and/or problems with RNA extraction reagents/kits (this is a ssRNA virus) as well as viral nucleic acid sequence for positive control (positive control is critical for this type of testing).
Re:US admits 'failing' on testing, says Fauci
by scrumbledober on Thursday March 12, 2020 @01:31PM
(#22559371)
we did PCR in my highschool biochem lab...
Re:US admits 'failing' on testing, says Fauci
by gitgudnubs on Thursday March 12, 2020 @02:19PM
(#22559916)
I've done it with an oven.