Re:CRISPR-Cas9 gene editing causes lots of mutations
by nonbel on Thursday July 19, 2018 @12:11PM
(#17567682)
>"current ex vivo therapies are unaffected since we can screen cells after editing."
If this is so easy then why is it not standard practice? From the paper:
>"Results reported here also illustrate a need to thoroughly examine the genome when editing is conducted ex vivo. As genetic damage is frequent, extensive and undetectable by the short-range PCR assays that are commonly used, comprehensive genomic analysis is warranted to identify cells with normal genomes before patient administration."
https://www.nature.com/articles/Nbt.4192
How much more expensive and time consuming will this procedure become if you require "comprehensive genomic analysis"? The merit of crispr/cas9 over other techniques was supposed to be how cheap and quick it was.
If this is so easy then why is it not standard practice? From the paper:
>"Results reported here also illustrate a need to thoroughly examine the genome when editing is conducted ex vivo. As genetic damage is frequent, extensive and undetectable by the short-range PCR assays that are commonly used, comprehensive genomic analysis is warranted to identify cells with normal genomes before patient administration." https://www.nature.com/articles/Nbt.4192
How much more expensive and time consuming will this procedure become if you require "comprehensive genomic analysis"? The merit of crispr/cas9 over other techniques was supposed to be how cheap and quick it was.