CRISPR-Cas9 gene editing causes lots of mutations

Re:CRISPR-Cas9 gene editing causes lots of mutations by zfrenchee on Thursday July 19, 2018 @11:57AM (#17567494)

Cas9 does not edit DNA, it cleaves DNA. What happens afterwards is up to the cell. The hype machine has ignored this fact.

One of my favorite George Church quotes: "CRISPR: some call it genome editing. I call it genome vandalism".

Base Editors can actually edit DNA, but only single bases at a time. http://www.sciencemag.org/news/2017/10/novel-crispr-derived-...

There's a big prize waiting for the person who can harness DNA repair pathways in conjunction with Cas9 to make precise, multi-base DNA edits. Lots of folks are working on that now.

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Re:CRISPR-Cas9 gene editing causes lots of mutations by 21 on Thursday July 19, 2018 @12:21PM (#17567785)

Does it cleave a whole segment off (two point cut), or just a single cut?

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Re:CRISPR-Cas9 gene editing causes lots of mutations by distant_hat on Saturday July 21, 2018 @02:51PM (#17583240)

Single cut.

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