Yes, because if there were such a list then you could assign a 1-to-1 mapping (by list index) of each number between 0 and 1 to a whole number which would prove they have the same size. If it is impossible to have a complete list of all real numbers between 0 and 1 then there cannot be such a mapping, and thus you'll "run out" of integers before you've counted all of the reals (more correctly you're showing that the size must be larger because if it was smaller or of equal size it could fit in such a list).

Numberphile did a pretty good introductory video to this if you want to learn more: https://www.youtube.com/watch?v=elvOZm0d4H0.