So, without going into too much detail the use of crispr/cas9 in our studies is even simpler than driving protein mutagenesis. We have been exploring CRISPRi has a mechanism to inhibit key promoter repressors of our gene of interest. We've found that we can affect affect specific protein abundances by changing the rates of both RNA synthesis and protein degradation, based on the two cross-kingdom control mechanisms CRISPRi and the N-end rule for protein stability.