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Re:Biomedical companies bleed 500k horseshoe crabs a year by saganus on Monday April 17, 2017 @12:32PM (#14132218)
Just for the tl;dr:

"Overall mortalities of bled horseshoe crabs were 17.8% (male = 18.75%; female = 17.2%), while mortalities of unbled crabs were 3.5%."

So it appears it indeed affects the crabs.

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