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Re:Yubikey with USB-C by epistasis on Thursday January 05, 2017 @12:50PM (#13329437)
The general protocol is U2F:

https://developers.yubico.com/U2F/

Yubico has some python code here:

https://github.com/Yubico/python-u2flib-host

https://github.com/Yubico/python-u2flib-server

Chrome builds U2F into the browser itself for website authentication.

Re:Yubikey with USB-C by emilecantin on Thursday January 05, 2017 @01:16PM (#13329696)
I've built a node.js version if Python is not your chosen stack: https://github.com/emilecantin/node-u2flib-server

Another choice that looks (sadly, for me) more popular: https://github.com/ashtuchkin/u2f