I'm a little more comfortable with saying we do some weighings in a vacuum (say, to determine the density of air at a given pressure) than with saying we'll start with a cylinder that contains a vacuum. For example, our 40kg cylinder, when airtight and containing vacuum, will weigh less than a 40kg object should, and I think that muddles the example.
Put in helium and you'll eventually reach and exceed the parity point as you keep filling up (same weight as the air), and the ratio of how much it moves for x volume of additional filled gas tells got the additional weight.
Soon enough you'll reach 2x air weight and you'll have tipped the scale exactly.
Granted, I was also using the first sense when talking about the weight of the helium specifically. I guess in that sense my terminology could have been better.
Suppose you have a balance scale with a "cylinder" full of air on one side. You pump out the air and put in helium. What happens to the scale?