Little's law doesn't imply what you said, either. It tells us the number of people in the system based on arrival and wait, but nothing about throughput.
And you're right about throughput, I was implicitly holding the number of people in the queue constant, and you can obviously add arbitrary delays and increase the number of people in queue without touching throughput.
Just like the website load times, you should be focused on the 99th percentile and the median. Single line reduces those metrics.
Really this boils down to whether the business is interested in saving a bit of money (fewer cashiers per purchase) or improving customer experience.
The metric you want is to figure out what wait time constitutes good/acceptable/bad service, and what percentage of people get that service.