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Re:Gene Editing Tool Hailed as a Breakthrough, and It Really... by entee on Tuesday December 29, 2015 @05:35PM (#10809532)
I've often wondered how CRISPR is actually useful, because at its core it's just a nuclease. It allows to you precisely cut DNA where you want to cut it. But for that to be useful you still have to put the DNA back together afterwards. I found this article useful for understanding the different ways that happens in practice:

https://www.addgene.org/CRISPR/guide/

Turns out you get some small mutations in the most common mechanism (non-homologous end joining or NHEJ). This could be a problem for use in therapies, but definitely not a huge issue for most research-based applications where you can control which mutants you allow to proceed to the next experiment.

Re:Gene Editing Tool Hailed as a Breakthrough, and It Really... by 88e282102ae2e5b on Tuesday December 29, 2015 @10:08PM (#10810466)
This is one reason why Cpf1 could be interesting, since it leaves sticky ends when it cuts: http://www.cell.com/cell/abstract/S0092-8674%2815%2901200-3