Re:Gene Editing Tool Hailed as a Breakthrough, and It Really...
by 88e282102ae2e5b on Tuesday December 29, 2015 @10:08PM
(#10810466)
This is one reason why Cpf1 could be interesting, since it leaves sticky ends when it cuts: http://www.cell.com/cell/abstract/S0092-8674%2815%2901200-3
https://www.addgene.org/CRISPR/guide/
Turns out you get some small mutations in the most common mechanism (non-homologous end joining or NHEJ). This could be a problem for use in therapies, but definitely not a huge issue for most research-based applications where you can control which mutants you allow to proceed to the next experiment.